Cell volume lab

Method and Materials:We calibrated the conduction sensor by measuring the conductivity when distilled water and 1.1% NaCl (338mOsm/kg) is added. We made sure that the conductivity probe powerful attached to the right insert on the SW500 Interface which is committed to the computer.

We took sm solely beaker to the movement of the room and we modify it ¾ full with the disposed(p) solution. We opened ?Lab Group? folder. We and so opened ? testing ground 2? folder. We clicked on the sensor. We accordingly(prenominal) opened the calibration segment of the program. We placed the conductivity probe first into distilled water and then we pressed ?take entering?. We inserted the submerging of distilled water in the table as 0mosm.

We opened the program for laboratory 2. We placed the probe in one of the 12 solutions. We started recording by activating the start button. We continued recording until the readings are durable then we clicked the ?stop? button. We habitd the ?smart tool? to read the condutivity value and then we entered the data in the table.

We removed the conductivity sensor from the beaker and then we shaked the sensor off. We made similar measurements of the rest 12 NaCl solutions, and then we entered the data into the data table in the row labeled conductivity. We saved the data file in our own group data folder. Next, we determined the osmolality of for each one of the 12 solutionsWe prepared test solutions; we used red grease pencil to label the 12 test thermionic valves from 1 to 11. We marked the eventually test tube-shaped structure with a letter ?C? refers to Control. From the front of the room, we placed 5 ml of 0% NaCl in the influence tube. We then placed 5 ml ranging in concentration from 0.1-1.1% NaCl into the other 11 tubes using pipettorWe Used the Eppendorf micropipet provided to add 20 l of blood to the tube. We attached the cap onto each tube and then mixed gently. We placed the circular test tube rack holding the 12 tubes into the provided water bath at 37OC for 30 minutes.

We mixed the tubes gently after incubation and then centrifuge the tubes for 5 minutes at 3000 RPM. We turned on the colorimeter; we next filled one of the cuvettes with distilled water to manage as fictitious character.



We pressed the ?Select? and ?Start/Stop? buttons at the same time. We inserted the reference cuvette and then pressed ?Select?. We waited until it verbalize ?CAL done?. We then opened the lid and removed the reference cuvette. We pressed ?Start? and began measuringWe transferred the solution gently using pipette from the top of the ?C into a clean rectangular cuvette so it is filled to within 0.5 cm from the top; we made sure to use a new pipette and a clean cuvette for each test tube measurement . We placed the cuvette into the Colorimeter and unappealing the lid. We read the %T (% Transmittance) value of the ?Control? tube and then recorded the value in the table. We left the Colorimeter lead to measure the %T value for cuvettes 1-10. After completing all measurements, we made sure to turn off the Colorimeter.

Bibliography:animal physiology, second eddition, Bulliet and Crossley

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