Unknown Lab Report

Unknown Lab idea: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 referable Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae thoughtless from the exhibitn #103 was a chiliad-negative Rod. The purpose of this science laboratory was to transgress and report the genus and species of an unknown bacterium. It is beta to depict an unknown micro- being because love how the bacterium work and how it is unified means knowing how it rouse affect humans. Unknown bacteria may similarly be employ clinically many an(prenominal) pharmaceutical drugs argon establish on products made by organisms (Katzung, B.G.2004). In ready to identify the unknown organism a series of tests were per organize. The tests use were glucose, sucrose, milk sugar fermentation; Urease, motility, MR-VP, citrate, oxidase, and indole these tests were used to identify the genus of the unknown and a McConkey agar-agar-agar scale leaf was used to differentiate and confirm the species of the unknown. This regurgitate will explain and give more in perspicacity reasons why certain tests were make and the results yielded from them. Materials & Methods On the first sidereal day after receiving the unknown micro-organism a streak exfoliation was aseptically done as shown in exercise 2 of the lad manual (Kleyn 9).

The streak home plate proficiency is done to fill a colony formed by a asterisk cell from a concoction containing millions of cells. The streak plate proficiency was also used to accommodate pure cultures of the bacteria development a trypticase soy agar (TSA) plate and was incubated at 37 ? for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the emplacement of the cells; if an organism appears purple on a lower floor a microscope then it is tell to be Gram positive, and if it appears remark then it is said to be Gram negative. To prevent the organism from encounterting contaminated,...If you want to get a full essay, dictate of battle it on our website: Ordercustompaper.com

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